This Procedure involves measurement of Creatine kinase activity in the presence of an antibody to CK-M monomer. this antibody completely inhibits the activity of CK-MM and half of CK-MB while not affecting the B subunit of CK-MB and CK-BB. Then IFCC reference method for measuring CK is used to Quantitatively determine CK-BB activity. CK catalyses the reaction between creatine phosphate and ADP giving creatine and ATP. ATP and glucose in the presence of G-PDH oxidises and reduces NAD to NADH. The rate of NADH formation is determined photometrically at 340 nm and is directly proportional to CK-BB activity. The CK-MB activity is calculated by multiplying CKBB by two.
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