SGOT (AST)

INTENDED USE
The test is applied for the quantitative determination
of AST activity in serum and plasma.
TEST PRINCIPLE
The enzyme aspartate aminotransferase (EC
2.6.1.1; L-Aspartate: 2-Oxoglutarate
Aminotransferase, AST or AspAT; Glutamate
Oxaloacetate Transaminase, GOT) catalyzes the
transaminase reaction between L-Aspartate and 2-
Oxoglutarate. The 2-Oxalacetate formed, is
reduced to malate in the presence of MDH. As the
reactions proceed, NADH is oxidized to NAD. The
disappearance of NADH per unit time is followed by
measuring the decrease in absorbance at 340 nm.