SGPT (ALT)

INTENDED USE
The test is applied for the quantitative determination
of GPT in serum.
TEST PRINCIPLE
The enzyme alanine aminotransferase (EC 2.6.1.2;
L-Alanine: 2-Oxoglutarate Aminotransferase, ALT
or A1aAT; Glutamate Pyruvate Transaminase,
GPT) catalyzes the transaminase reaction between
L-Alanine and 2-Oxoglutarate. The pyruvate
formed, is reduced to lactate in the presence of
LDH. As the reactions proceed, NADH is oxidized
to NAD. The disappearance of NADH per unit time
is followed by measuring the decrease in
absorbance at 340 nm.