TRIGLYCERIDES

INTENDED USE
The test is applied for quantitative determination of
Triglycerides concentration in serum or EDTA
plasma.
TEST PRINCIPLE
Triglycerides are hydrolized by lipoproteinlipase to
produce glycerol and free fatty acids. The glycerol
participates in a series of coupled enzymatic
reactions, in which glycerol kinase / glycerol
phosphate oxidase are involved and H2O2 is
generated. The H2O2 reacts with p-chlorophenol
and 4-aminoantipyrine in the presence of peroxidase
to form a quinoneimine dye. The intensity of color
formed is proportional to the triglycerides
concentration and can be measured photometrically
between 480 and 520 nm.